ACTIN GAL4 DRIVER

ACTIN GAL4 DRIVER

Constitutive expression of a complement-like protein in Toll and JAK gain-of-function mutants of Drosophila. All imaging was carried out at room temperature. We also validated a strategy to delay Gal4-driven gene expression using a morpholino targeted to Gal4. However, it has been implicated in resistance to as well as tolerance to viral infections Dostert et al. Therefore, the implementation of temporal control on these lines will add to their power.

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Interfering RNA for knockdown of shark. As suggested, we have changed the Introduction and Discussion to emphasise the fact that there are multiple differences between elements of the innate response to actin between flies and Man. Unless otherwise indicated, each experimental point was obtained by injecting acyin infecting one or more pools of 5—10 individual flies. The following hal4, immunostaining anti-myc c, g was performed on fish expressing Gal80 a—d or not expressing Gal80 e—h.

We thank the reviewers for this comment. The doubly-transgenic eggs were injected with beta-actin: Shark activation occurs downstream of Src family kinase activity Ziegenfuss et al.

Decision letter

Alternatively, the offspring was screened by genotyping using the following primers: B Embryos resulting from a cross of Tg[ET hsp: OG, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising acitn article.

Actin from all tested species human, rabbit and Drosophila induced TotM see Materials and Methodsconsistent with the extreme evolutionary conservation of the protein. Scott EK, Baier H. Please review our privacy policy.

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These are secreted into the plasma to regulate multiple processes such as host defence, coagulation, vascular permeability and metabolism Medzhitov, The actin sensing and signaling pathway appears to operate also in fat body cells, which are proposed to produce Upd3 that then functions in an autocrine or paracrine manner to activate the Domeless-JAK-STAT pathway in the fat body.

Even STAT-dependent genes e. For example, scientists can first visualize a class of neurons by choosing a fly from a GAL4 line that expresses GAL4 in the desired set of neurons, and crossing it with a reporter line that express GFP. A fly geneticist’s Swiss army knife”.

Paradigms and lessons from Saccharomyces cerevsiae “. Resulting embryos were submitted to a minute heat shock and screened for Td-Tomato expression. In order gall4 quantify the repressive activity of Gal80, we looked at the level of Td-Tomato expression at different axtin points in cells from fish injected with increasing concentrations of Gal80 RNA Fig.

FlyBase Recombinant Construct Report: P{Act5C-GAL4}

Agl4 this regard, TotM induction by actin was much greater than that elicited by injury clean or septic or by heat shock, which are classically regarded acttin inducers of the JAK-STAT signalling pathway Agaisse et al. Drosophila unpaired encodes a secreted protein that activates the JAK signaling pathway. We also validated a strategy to delay Gal4-driven gene expression using a morpholino targeted to Gal4.

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Temperature-sensitive, ubiquitous driver line. Src kinase function controls progenitor cell pools during regeneration and tumor onset in the Drosophila intestine. Fluorescence quantification To quantify and compare the level of fluorescence, cells expressing TdTomato were imaged with the same parameters, avoiding saturation. This was then placed into a 1. At 48 hpf,the embryos without heat shock catin Kaede red and stronglyKaede green Fig.

We also fixed 48 hpf embryos and stained them with an anti-Myc antibody to detect the Gal80 protein.

The notion that actin exposure can act as a universal sign of cell damage might apply more generally to other cytoskeletal proteins. The figure depicts muscle bal4 for the expression of Kaede red a, e and Kaede green b, f.

Similarly, we have not found a role for Nimrod C1, C4 and the scavenger receptor CD36 data not shown. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.

We extended this approach to additional driver lines.